Comments on: HiSeq 2000

By: Tech Cheat Sheet Nasdaq Analysis and Stocks to Watch: Google (GOOG) EMC (EMC), ValueClick (VCLK), Illumina (ILMN) | Wall St. Cheat Sheet

Mon, 21 Jun 2010 14:18:03 +0000

[...] earnings front, having announced a breakthrough new sequencing platform during the first quarter—the HiSeq2000—and handily beating analyst estimates on both revenues and earnings per share. Illumina’s [...]

By: dd

dd — Tue, 16 Mar 2010 23:39:02 +0000

Daniel, an index run is a multiplex/barcoded run. I don’t like the term barcode for this because the analogy is weak. My point was that only the chemistry/acquisition cycles need to be correlated, not read lengths, not run types, not cycles, etc.

By: Daniel

Daniel — Tue, 16 Mar 2010 18:23:50 +0000

What do you mean when you say, “…even do an indexing run on one flow cell and a standard paired-end run on the other”. I understand the theory of off-setting the two flowcells (one is in chemistry, while the other is imaging), but what exactly is an indexing run. The only context I know of “indexing” is for reading the barcode of multiplexed samples.

Thanks for all the info.

By: dd

dd — Wed, 17 Feb 2010 22:28:06 +0000

Keith Robison, sorry for not responding sooner (I meant to). Even Illumina GA flow cells had clusters on the top and bottom. There is nothing magical about any of the surfaces on the flow cell, they all have grafting oligos on them and they all have clusters. Illumina has just figured out how to image both surfaces.

By: Genome Sequencing Under $10,000 « ScienceRoll

Genome Sequencing Under $10,000 « ScienceRoll — Sun, 17 Jan 2010 12:14:40 +0000

[...] PolITiGenomics [...]

By: Another Stop on the Road to the $1,000 Genome

Another Stop on the Road to the $1,000 Genome — Wed, 13 Jan 2010 19:58:20 +0000

[...] 1/13: Daniel MacArthur of Genetic Future, Luke Jostins of Genetic Interference and David Dooling of PolITiGenomics all offer their analysis of what Illumina’s announcement means for the present and future of [...]

By: The Future of Second Generation Sequencing « Genetic Inference

The Future of Second Generation Sequencing « Genetic Inference — Wed, 13 Jan 2010 12:51:41 +0000

[...] machine: for that, see posts at Genomics Law Report, Genome Web, Genetic Future, Pathogenomics and PolITiGenomics. What I really care about is what this machine implies for the future of sequencing, and [...]

By: Pathogens: Genes and Genomes » New Illumina Announced – BGI to become world’s largest genome centre

Wed, 13 Jan 2010 09:33:12 +0000

[...] I won’t cover this in detail as there are already great blog posts up from Daniel McArthur, David Dooling and Genomics Lawyer. Bottom-line: $690,000 for the machine, $10,000 for the reagents, 2 x 100 [...]

By: Keith Robison

Keith Robison — Wed, 13 Jan 2010 03:14:26 +0000

Thanks for all the detailed information!!!

The one thing I’m having a little challenge wrapping my head around is the shooting the flow cells from both top and bottom. Is there really depth to the cluster generation? Does the new flowcell really have two active surfaces in it?