NGS Informatics

Below is a table with informatics and IT statistics for the major next-generation/massively parallel sequencing platforms. The information in the table is approximate and should only be used for general, informational purposes.

Next-Generation Sequencing Statistics

Vendor: Roche Illumina ABI
Technology: 454 Solexa GA SOLiD
Platform: GS20 FLX Ti I II IIx 1 2 3
Reads: (M) 0.5 0.5 1.25 28 100 250 40 115 320
Fragment
Read length: 100 200 400 35 50 100 25 35 50
Run time: (d) 0.25 0.3 0.4 3 3 5 6 5 8
Yield: (Gb) 0.05 0.1 0.5 1 5 25 1 4 16
Rate: (Gb/d) 0.2 0.33 1.25 0.33 1.67 5 0.34 1.6 4
Images: (TB) 0.01 0.01 0.03 0.5 1.1 2.8 1.8 2.5 1.9
PA Disk: (GB) 3 3 15 175 300 300 300 750 1200
PA CPU: (hr) 10 140 220 100 70 NA NA NA NA
SRA: (GB) 0.5 1 4 30 50 2.5 100 140 600
Paired-end
Read length: 200 400 2×35 2×50 2×100 2×25 2×35 2×50
Insert: (kb) 3.5 3.5 0.2 0.2 0.2 3 3 2
Run time: (d) 0.3 0.4 6 10 10 12 10 16
Yield: (Gb) 0.1 0.5 2 9 50 2 8 32
Rate: (Gb/d) 0.33 1.25 0.33 1.67 5 0.34 1.6 4
Images: (TB) 0.01 0.03 1 2.2 5.6 3.6 5 3.8
PA Disk: (GB) 3 15 350 500 550 600 1500 2400
PA CPU: (hr) 140 220 160 120 NA NA NA NA
SRA: (GB) 1 4 60 100 3.5 200 280 1200

Notes:

  • Units: B - bytes, b - bases
  • PA is primary analysis (includes image feature extraction and base calling)
  • PA CPU is calculated as the wall clock multiplied by the number of CPU cores
  • ABI SOLiD data, except rate, are representative of a single slide
  • ABI SOLiD and Illumina GA IIx primary analysis is done on instrument
  • 454 paired-end reads vary in length depending on location of internal adapter
  • SRA is the size of the files (SFF, SRF, or FASTQ) that are submitted to the NCBI Short Read Archive