Below is a table with informatics and IT statistics for the major next-generation/massively parallel sequencing platforms. The information in the table is approximate and should only be used for general, informational purposes.
Next-Generation Sequencing Statistics
| Vendor: | Roche | Illumina | ABI | |||||
|---|---|---|---|---|---|---|---|---|
| Technology: | 454 | Solexa | SOLiD | |||||
| Platform: | GS 20 | FLX | Ti | GA | GA II | 1 | 2 | |
| Reads: | 500 k | 500 k | 1 M | 28 M | 80 M | 40 M | 115 M | |
| Fragment | ||||||||
| Read length: | 100 | 200 | 350 | 35 | 50 | 75 | 25 | 35 |
| Run time: | 6 hr | 7 hr | 9 hr | 3 d | 3 d | 4 d | 6 d | 5 d |
| Yield: | 50 Mb | 100 Mb | 400 Mb | 1 Gb | 4 Gb | 6 Gb | 1 Gb | 4 Gb |
| Images: | 11 GB | 13 GB | 27 GB | 500 GB | 1.1 TB | 1.7 TB | 1.8 TB | 2.5 TB |
| PA Disk: | 3 GB | 3 GB | 15 GB | 175 GB | 300 GB | 350 GB | 300 GB | 750 GB |
| PA CPU: | 10 hr | 140 hr | 220 hr | 100 hr | 70 hr | 100 hr | NA | NA |
| SRA: | 500 MB | 1 GB | 4 GB | 30 GB | 50 GB | 75 GB | 100 GB | 140 GB |
| Paired-end | ||||||||
| Read length: | 200 | 2×35 | 2×50 | 2×75 | 2×25 | 2×35 | ||
| Insert: | 3.5 kb | 200 b | 200 b | 200 b | 3 kb | 3 kb | ||
| Run time: | 7 hr | 6 d | 6 d | 8 d | 12 d | 10 d | ||
| Yield: | 100 Mb | 2 Gb | 8 Gb | 11 Gb | 2 Gb | 8 Gb | ||
| Images: | 13 GB | 1 TB | 2.2 TB | 3.4 TB | 3.6 TB | 5 TB | ||
| PA Disk: | 3 GB | 350 GB | 500 GB | 600 GB | 600 GB | 1.5 TB | ||
| PA CPU: | 140 hr | 160 hr | 120 hr | 170 hr | NA | NA | ||
| SRA: | 1 GB | 60 GB | 100 GB | 150 GB | 200 GB | 280 GB | ||
Notes:
- Units: B - bytes, b - bases
- PA is primary analysis (includes image feature extraction and base calling)
- PA CPU is calculated as the wall clock multiplied by the number of CPU cores
- ABI SOLiD data are representative of a single slide
- ABI SOLiD primary analysis is done on the instrument cluster
- SRA is the size of the files (SFF or SRF) that are submitted to the NCBI Short Read Archive
